TY - BOOK AU - Valeur,Bernard AU - Berberan-Santos,M.N. TI - Molecular fluorescence :principles and applications SN - 9783527328468 (pbk): AV - QC477 .V35 2012 U1 - 543.56 23rd PY - 2013/// CY - Germany PB - Wiley-VCH KW - Fluorescence KW - Chemistry KW - Fluorescence spectroscopy KW - Fluorescence microscopy KW - Fluorescent probes KW - Molecular spectroscopy KW - Molecular probes KW - fast N1 - Previous edition: 2002; Includes bibliographical references and index; Introduction -- Principles: Absorption of ultraviolet, visible and near-infrared radiation ; Characteristics of fluorescence emission ; Structural effects on fluorescence emission ; Environmental effects on fluorescence emission ; Effects of intermolecular photophysical processes on fluorescence emission ; Fluorescence polarization : emission anisotropy ; Excitation energy transfer -- Techniques: Steady-state specrofluorometry ; Time-resolved fluorescence techniques ; Fluorescence microscopy ; Fluorescence correlation spectroscopy and single-molecule fluorescence spectroscopy -- Applications: Evaluation of local physical parameters by means of fluorescent probes ; Chemical sensing via fluorescence -- Autofluorescence and fluorescence labeling in biology and medicine ; Miscellaneous applications N2 - This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most signifi cant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology. ER -