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040			_cIISER-BPR
041			_aENG
082			_a543.67 _bMOB _223rd
100			_aMobius, Klaus
222			_aChemistry
245		0	_aHigh-field EPR spectroscopy on proteins and their model systems : _bCharacterization of transient paramagnetic states
250			_a1st ed.
260			_aCambridge: _bRSC Publishing, _cc2009.
300			_axvi, 375p. : _bill(col.), hbk. ; _c24cm
520			_aUnderstanding the major factors determining the specificity of transmembrane transfer processes in proteins is now a hot topic in molecular bio-science. Advanced electron paramagnetic resonance (EPR) at high magnetic fields is a powerful technique for characterizing the transient states of proteins in action. High-Field EPR Spectroscopy on Proteins and their Model Systems: characterization of Transient Paramagnetic States offers a comprehensive overview of experimental techniques in, and paradigmatic examples of, the application of high-field EPR spectroscopy in biology and chemistry. The book’s focus is on the use of the technique in conjunction with site-specific mutation strategies and advanced quantum-chemical computation methods to reveal protein structure and dynamics. This yields new insights into biological processes at the atomic and molecular level. The theoretical and instrumental background of high-field EPR is described and examples of paradigmatic protein systems, such as photosynthetic reaction centres, are discussed in the light of recent investigations. Aspects of structure dynamics-function relations that are revealed by studying site-specific mutants are highlighted, thereby combining high-field EPR with genetic engineering techniques. The information obtained complements that obtained from protein crystallography, solid-state NMR, infrared and optical spectroscopy. The book documents both background knowledge and results of the latest research in the field. Unique features include comparisons of information content of EPR, ENDOR, Triple resonance, ESEEM and PELDOR taken at different microwave frequencies and magnetic fields. Coherent treatment of the subject by the leading Berlin high-field EPR laboratory covers the theoretical background as well as state-of-art research both in terms of instrumentation and application to biological systems. The book provides an outlook to future developments and references for further reading and is essential reading for postdoctoral scientists, professionals, academics and graduate students working in this field. Also includes colored illustrations, bibliographic references and subject index.
650			_aChemistry
650			_aAnalytical Chemistry
650			_aElectron Paramagnetic Resonance Spectroscopy
700			_aSavitsky, Anton
942			_cBK _2ddc
942			_2ddc
947			_a14922.3708
948			_a0.22
999			_c4166 _d4166